Positive Health Online

Abstract

AGUIRRE and COLLEAGUES, 1. Department of Physiological Sciences, College of Veterinary Medicine, University of Florida (UF), Gainesville, FL 32610, United States. : aguirrej@ufl.edu ; 2. Osteoporosis Research Center, School of Medicine, Creighton University, Omaha, NE 68131, United States.  mohammedakhter@creighton.edu ; 3. Department of Physiological Sciences, College of Veterinary Medicine, University of Florida (UF), Gainesville, FL 32610, United States.  kgncfl27@yahoo.com 4. Department of Physiological Sciences, College of Veterinary Medicine, University of Florida (UF), Gainesville, FL 32610, United States.  trcalekcarley@ufl.edu ; 5. Department of Physiological Sciences, College of Veterinary Medicine, University of Florida (UF), Gainesville, FL 32610, United States.  aleeper@ufl.edu ; 6. Department of Physiological Sciences, College of Veterinary Medicine, University of Florida (UF), Gainesville, FL 32610, United States.  williamsaly@ufl.edu ; 7. Department of Periodontology and Oral Biology, College of Dentistry, UF, United States.: mrivera@dental.ufl.edu ; 8. Department of Periodontology and Oral Biology, College of Dentistry, UF, United States; Bone Research, Amgen Inc., Thousand Oaks, CA 91320, United States. : kesavalu@dental.ufl.edu  9. Bone Research, Amgen Inc., Thousand Oaks, CA 91320, United States.  huazhu.ke@ucb.com ; 10. Bone Research, Amgen Inc., Thousand Oaks, CA 91320, United States. minl@amgen.com ; 11. Department of Physiological Sciences, College of Veterinary Medicine, University of Florida (UF), Gainesville, FL 32610, United States.  kimmeldb@comcast.net  characterized in rice rats: (a) periodontitis (PD) progress with feeding of standard laboratory rat chow (STD) during ages 4-80 weeks; and (b) PD progress with feeding of a high sucrose-casein (H-SC) diet during young adulthood.

Background

To characterize in rice rats: (a) periodontitis (PD) progress with feeding of standard laboratory rat chow (STD) during ages 4-80 weeks; and (b) PD progress with feeding of a high sucrose-casein (H-SC) diet during young adulthood.

Methodology

One group (N=12) was euthanized at age 4 weeks (Baseline). Four groups (N=8-16) consumed a STD diet from baseline and were necropsied at ages 22, 30, 52, and 80 weeks. Three groups (N=10-16) consumed an H-SC diet from baseline. Two were necropsied at ages 22 and 30 weeks, respectively. The third switched to the STD diet at age 22 weeks and was necropsied at age 30 weeks. All mandibles/maxillae were assessed by histometry for degree of periodontal inflammation (PD Score), alveolar crest height (ACH, mm), and horizontal alveolar bone height (hABH, mm2).

Results

In STD diet rats aged ≥30 weeks, all endpoints were worse (P<0.05) than at Baseline. In H-SC diet rats aged ≥22 weeks, all endpoints were worse than at Baseline (P<0.05). At age 22 weeks, all endpoints were worse in the H-SC group than in the STD group (P<0.05). By age 30 weeks, the STD and H-SC groups did not differ.

Conclusion

1) STD diet fed rice rats develop moderate/severe PD by age 30 weeks; 2) an H-SC diet accelerates moderate/severe PD development; and 3) switching to a STD diet does not halt/reverse PD that was accelerated by an H-SC diet. These data further clarify use of the rice rat as a PD model.

References

Aguirre JI1, Akhter MP2, Neuville KG3, Trcalek CR4, Leeper AM5, Williams AA6, Rivera M7, Kesavalu L8, Ke HZ9, Liu M10, Kimmel DB11. Age-related periodontitis and alveolar bone loss in rice rats. Arch Oral Biol.  ;73:193-205. doi: 10.1016/j.archoralbio.2016.10.018. Epub Oct 17 2016 . Jan 2017.