Positive Health Online

Abstract

CAPÓ and COLLEAGUES, 1. Research Group on Community Nutrition and Oxidative Stress, Science Laboratory of Physical Activity, Department of Fundamental Biology and Health Sciences, University of Balearic Islands, Crtra. Valldemossa, km 7.5, E-07122 Palma de Mallorca, Illes Balears Spain ; CIBER: CB12/03/30038 Pathophysiology of Obesity and Nutrition, CIBEROBN, Health Institute Carlos III (ISCIII), University of Balearic Islands, 07122 Palma de Mallorca, Spain; 2. Research Group on Community Nutrition and Oxidative Stress, Science Laboratory of Physical Activity, Department of Fundamental Biology and Health Sciences, University of Balearic Islands, Crtra. Valldemossa, km 7.5, E-07122 Palma de Mallorca, Illes Balears Spain ; Department of Nutrition and Dietetics, Faculty of Pharmacy, University of Concepción, 4070386 Concepción, Chile evaluated the effects of training, exercise and docosahexaenoic (DHA) supplementation on plasma eicosanoids levels and peripheral blood mononuclear cells (PBMCs) eicosanoids production in footballers.

Background

Eicosanoids mediate initiation and resolution of inflammation. Our aim was evaluating the effects of training, exercise and docosahexaenoic (DHA) supplementation on plasma eicosanoids levels and peripheral blood mononuclear cells (PBMCs) eicosanoids production.

Methodology

Fifteen male footballers were distributed to placebo and experimental groups. Experimental group consumed DHA-enriched beverage (1.16 g DHA/day) for 8 weeks, placebo group consumed a placebo beverage. Blood samples were taken before and after the nutritional intervention in basal conditions and 2 h after acute exercise.

Results

Training increased basal Prostaglandin E1 (PGE1) plasma levels and PBMCs cyclooxygenase 2 (COX-2) protein levels in both groups, but COX-1 protein levels only in the experimental group. Acute exercise increased plasma PGE2 and PBMCs active NFκβ levels. Lipopolysaccharide (LPS)-stimulated PBMCs increases eicosanoids production (PGE1, PGE2, RvD1) in both groups and increased LPS-stimulated PBMCs active NFκβ. DHA supplementation increased COX-2 levels but decreased LPS-stimulated PBMCs PGE1 and PGE2 production. Neither DHA supplementation nor acute exercise altered the expression of NFκβ, COX-2, 15-LOX2, 5-LOX, or IL-1β genes in PBMCs.

Conclusion

The increase of PGE1 plasma levels after training promoted systemic anti-inflammatory and vasodilator environment. Exercise and DHA supplementation acted synergistically by increasing plasma PGE2 with anti-inflammatory effects. Exercise primed PBMCs to enhance PGE1, PGE2 and RvD1 production in response to LPS. Trial Registration: The project was registered at ClinicalTrial.gov (NCT02177383).

References

Capó X1, Martorell M2, Sureda A1, Tur JA1, Pons A1. Effects of dietary Docosahexaenoic, training and acute exercise on lipid mediators. J Int Soc Sports Nutr. 13:16. doi: 10.1186/s12970-016-0126-y. Apr 5 2016. eCollection 2016.