Positive Health Online

Abstract

CHEN and COLLEAGUES, 1. Department of Medical Genetics, China Medical University, Shenyang, 110122, P.R. China; 2. Department of Laboratory Medicine, No. 202 Hospital of PLA, Shenyang, 110003, P.R. China; 3. Department of Otolaryngology, No. 463 Hospital of PLA, Shenyang, 110007, P.R. China explored the role and molecular mechanism of miR-27a in laryngeal cancer differentiation in the study

Background

miR-27a regulates cell differentiation in a variety of diseases. However, whether and how miR-27a participates in laryngeal cancer cell differentiation remains unknown.

Methodology

The authors explored role and molecular mechanism of miR-27a in laryngeal cancer differentiation in the study.

Results

The authors found that miR-27a expression was inversely correlated with laryngeal cancer differentiation degree based on the clinical pathological diagnosis of each patient. miR-27 significantly rescued differentiation and inhibited β-catenin, LEF1, OCT4 and SOX2 in Wnt/β-catenin pathway in all-trans-retinoic acid (ATRA)-induced laryngeal cancer cells. Bindings of RARα to miR-27a and miR-27a to GSK-3β were confirmed by ChIP and Luciferase reporter assays, respectively.

Conclusion

In conclusion, miR-27a is a negative regulator in laryngeal cancer differentiation. RARα-mediated miR-27a transcriptional inactivation releases the inhibition of miR-27a on GSK-3β leading to laryngeal cancer differentiation through GSK-3β-involved Wnt/β-catenin pathway, suggesting that miR-27a is a usefully therapeutic target at least in ATRA-induced laryngeal cancer differentiation.

References

Chen S1, Sun YY1, Zhang ZX1, Li YH2, Xu ZM3, Fu WN1. Transcriptional suppression of microRNA-27a contributes to laryngeal cancer differentiation via GSK-3β-involved Wnt/β-catenin pathway Oncotarget;8(9):14708-14718. doi: 10.18632/oncotarget.14769. 28 Feb 2017