Positive Health Online

Abstract

DANIELS and COLLEAGUES, (1)Centre for Public Health, Queen's University Belfast, Pathology Building, Grosvenor Road, Belfast BT12 6BJ, UK. j.mceneny@qub.ac.uk studied if increased fruit and vegetable (F&V) consumption influenced the carotenoid content and enzymes associated with the antioxidant properties of HDL in subjects with type 2 diabetes (T2D).

Background

High density lipoproteins (HDL) have many cardioprotective roles; however, in subjects with type 2 diabetes (T2D) these cardioprotective properties are diminished. Conversely, increased fruit and vegetable (F&V) intake may reduce cardiovascular disease risk, although direct trial evidence of a mechanism by which this occurs in subjects with T2D is lacking. Therefore, the aim of this study was to examine if increased F&V consumption influenced the carotenoid content and enzymes associated with the antioxidant properties of HDL in subjects with T2D.

Methodology

Eighty obese subjects with T2D were randomized to a 1- or ≥6-portion/day F&V diet for 8-weeks. Fasting serum was collected pre- and post-intervention. HDL was subfractionated into HDL2 and HDL3 by rapid ultracentrifugation. Carotenoids were measured in serum, HDL2 and HDL3 by high performance liquid chromatography. The activity of paraoxonase-1 (PON-1) was measured in serum, HDL2 and HDL3 by a spectrophotometric assay, while the activity of lecithin cholesterol acyltransferase (LCAT) was measured in serum, HDL2 and HDL3 by a fluorometric assay.

Results

In the ≥6- vs. 1-portion post-intervention comparisons, carotenoids increased in serum, HDL2 and particularly HDL3, (α-carotene, p = 0.008; β-cryptoxanthin, p = 0.042; lutein, p = 0.012; lycopene, p = 0.016), as did the activities of PON-1 and LCAT in HDL3 (p = 0.006 and 0.044, respectively).

Conclusion

To the authors’ knowledge, this is the first study in subjects with T2D to demonstrate that increased F&V intake augmented the carotenoid content and influenced enzymes associated with the antioxidant properties of HDL. The authors suggest that these changes would enhance the cardioprotective properties of this lipoprotein. Clinical Trial Registration: ISRCTN21676269.

References

Daniels JA, Mulligan C, McCance D, Woodside JV, Patterson C, Young IS, McEneny J(1). A randomised controlled trial of increasing fruit and vegetable intake and how this influences the carotenoid concentration and activities of PON-1 and LCAT in  HDL from subjects with type 2 diabetes. Cardiovasc Diabetol. 13:16. Jan 14 2014. doi: 10.1186/1475-2840-13-16.

Comment

The above study is the first study to demonstrate that increasing fruit and vegetable intake enhance the antioxidant properties of High density lipoproteins (HDL) which have cardioprotective function for people with Type 2 diabetes.