Positive Health Online

Abstract

FERRARI DE ANDRADE and COLLEAGUES, Universidade Federal do Paraná(UFPR), Brazil,  Institute Pasteur, France, Queensland Institute of Medical Research, Australia, andPontifícia Universidade Católica, Paraná, Brazil assessed the in vivo antitumor potential of a highly diluted natural complex named M-1.

Background

Malignant melanoma is a lethal skin melanocytic neoplasm that forms metastasis to distant organs. Inhibition of tumour-related angiogenesis results in decreased nutrient transport subsequently promoting tumour hypoxia. The aim of this study was to assess the in vivo antitumor potential of a highly diluted natural complex named M-1. M-1 composition is based on the following natural matrixes in Hahnemann decimal dilutions (dH): Aconitum napellus (20dH), Arsenicum album (18dH), Asa foetida (20dH), Calcarea carbonica (16dH), Chelidonium majus (20dH), Cinnamon (20dH), Conium maculatum (17dH), Echinacea purpurea (20dH), Gelsemium sempervirens (20dH), Ipecacuanha (13dH), Phosphorus (20dH), Rhus toxicodendron (17dH), Silicea (20dH), Sulphur (24dH), and Thuja occidentalis (19dH). Thus anti-angiogenic strategies offer real potential for future therapeutics. Pre-clinical and clinical studies have described new targets and approaches for identifying significant parameters involved in angiogenesis inhibition. The aim of this study was to assess the in vivo antitumor potential of a highly diluted natural complex named M-1.

Methodology

B16-F10 cells were subcutaneously injected on dorsal flank of C57BL/6 mice. After 24 hours of B16-F10 injection, M-1 treatment was administered for a period of 10 minutes with a modified inhalation chamber. Subsequent treatments occurred twice a day for 14 days. After this treatment period, mice were weighed and euthanized, then solid tumours were removed, weighed and measured (all animals developed melanoma tumours). The tumours were imaged and processed for histopathology by Fontana-Masson staining. Slides were analyzed using an automated slide scanner, Mirax Scan (Carl ZeissTM). Final images were analyzed using Mirax Viewer Software (3DHISTECHTM).

Results

Visual inspection of tumours showed an obvious decrease in tumour size, confirmed by wet weight measurement indicating tumour size to be reduced by 38%, whereas mice body weight was unaffected. When analyzed by histology, tumour area from M-1 treated mice was decreased, albeit with low significance .

Conclusion

In summary, treatment with the highly diluted natural complex M-1 resulted in decrease in tumour size and weight. It is important to notice M-1 in vivo anticancer action, but more interesting is the first demonstration of a non-invasive route of therapy for cancer: the inhalation. Despite the promise of these series of experiments, further investigation on M-1 mechanism of action and its biochemical properties is necessary to develop more efficient therapies.

References

Lucas Ferrari de Andrade, Stellee Marcela Petris Biscaia, Gustavo Rossi, Aline Raquell Leck, Cleber Rafael Vieira da Costa, Brian Mozeleski, Fernando de Souza Fonseca Guimarães, Rafael Zotz, Juarez Gabardo,Carolina Camargo de Oliveira, Dorly de Freitas Buchi, Edvaldo da Silva Trindade. Highly diluted natural complex of homeopathic medicines inhibits melanoma growth in vivo. Int J High Dilution Res 12(44):95-97. 2013. Proceedings of the XXVII GIRI Symposium; 03-04; Bern(Switzerland). Sep 2013.

Comment

This research demonstrates the decrease in melanoma tumour size and weight in vivo in mice using a dilute complex of homeopathic medicines delivered via inhalation. If replicated, this points to a highly promising avenue for treatment of lethal melanoma.