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Research: SANG and COLLEAGUES,
Listed in Issue 212
Abstract
SANG and COLLEAGUES, Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, P.R. China; Institute of Hydrobiology, Ji' nan University, Guangzhou 510632, P.R.China studied the generation of superoxide radical (O2.-) in Cyt b6f of Bryopsis corticulans under high light illumination using electron paramagnetic resonance (EPR) spectroscopy.
Background
Methodology
The generation of superoxide radical (O2.-) in Cyt b6f of Bryopsis corticulans under high light illumination was studied using electron paramagnetic resonance (EPR) spectroscopy. This could be evidenced by the addition of SOD which specifically reacted with O2.-.
Results
The generation of O2.- was lost in the absence of oxygen and was found to be suppressed in the presence of NaN3 and be scavenged by extraneous antioxidants such as ascorbate, beta-carotene and glutathione which could also scavenged 1O2*. These results indicated that O2.- which produced under high light illumination in Cyt b6f of B. corticulans might rise from a reaction which 1O2* could participated in. Also the photo-protection mechanism to Cyt b6f complex by antioxidants which might contain in thylakoid was speculated.
Conclusion
References
Sang M, Xie J, Qin XC, Wang WD, Chen XB, Wang KB, Zhang JP, Li LB and Kuang TY. High-light induced superoxide radical formation in cytochrome b6f complex from Bryopsis corticulans as detected by EPR spectroscopy. Journal of Photochemistry & Photobiology. B - Biology. 102(3):177-81. Mar 2 2011.
