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Research: SHAKHSSALIM and COLLEAGUES,
Listed in Issue 216
Abstract
SHAKHSSALIM and COLLEAGUES, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran. massoudh@nigeb.ac.ir used DNA from bladder cancer and control patients to investigate sequence variation in the mitochondrial D-loop region.
Background
Bladder cancer is a relatively common and potentially life-threatening neoplasm that ranks ninth in terms of worldwide cancer incidence. The aim of this study was to determine deletions and sequence variations in the mitochondrial displacement loop (D-loop) region from the blood specimens and tumoural tissues of patients with bladder cancer, compared to adjacent non-tumoural tissues.
Methodology
The DNA from blood, tumoural tissues and adjacent non-tumoural tissues of twenty-six patients with bladder cancer and DNA from blood of 504 healthy controls from different ethnicities were investigated to determine sequence variation in the mitochondrial D-loop region using multiplex polymerase chain reaction (PCR), DNA sequencing and southern blotting analysis.
Results
From a total of 110 variations, 48 were reported as new mutations. No deletions were detected in tumoural tissues, adjacent non-tumoural tissues and blood samples from patients. Although the polymorphisms at loci 16189, 16261 and 16311 were not significantly correlated with bladder cancer, the C16069T variation was significantly present in patient samples compared to control samples (p<0.05). Interestingly, there was no significant difference (p>0.05) of C variations, including C7TC6, C8TC6, C9TC6 and C10TC6, in D310 mitochondrial DNA between patients and control samples.
Conclusion
Our study suggests that 16069 mitochondrial DNA D-Loop mutations may play a significant role in the aetiology of bladder cancer and facilitate the definition of carcinogenesis-related mutations in human cancer.
References
Shakhssalim N, Houshmand M, Kamalidehghan B, Faraji A, Sarhangnejad R, Dadgar S, Mobaraki M, Rosli R and Sanati MH. The mitochondrial C16069T polymorphism, not mitochondrial D310 (D-loop) mononucleotide sequence variations, is associated with bladder cancer. Cancer Cell International. 13(1):120. 2013. Other ID. NLM. PMC3930351. 2013.
