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Research: BOUMERFEG and ARRAR,
Listed in Issue 166
Abstract
BOUMERFEG and ARRAR, Laboratory of Applied Biochemistry, Department of Biology, Faculty of Sciences, University Ferhat Abbas of Setif 19000, Algeria studied the antioxidant properties of Black Bryony (Tamus communis), traditionally used in Algerian folk medicine.
Background
This study was conducted to search for xanthine oxidase (XO) inhibitors from the root extracts of Tamus communis L. traditionally used in folk medicine in Algeria.
Methodology
Root extracts with different solvents were screened for purified milk xanthine oxidase inhibition.
Results
The root extracts (methanol, chloroform and ethyl acetate) and proteins, obtained in distilled water, inhibited bovine, sheep and human milk XO from three species in a concentration-dependent manner, with an additional superoxide scavenging capacity, which reached its highest level with ethyl acetate extract (IC(50) = 0.15, 0.04 and 0.09 g/L) for bovine XO, sheep XO and human XO, respectively. The antioxidant potential was confirmed with the non-enzymatic method, total radical-trapping antioxidant parameter (TRAP) assay, which showed that the Tamus communis L. extracts have a potential antioxidant activity in the same order obtained by using the reduction of cytochrome c, an enzymatic method, in which the antioxidant activity followed a decreasing order: ethyl acetate extract > chloroform extract > protein. Copyright (c) 2008 John Wiley & Sons, Ltd.
Conclusion
References
Boumerfeg S, Baghiani A, Messaoudi D, Khennouf S and Arrar L. Antioxidant properties and xanthine oxidase inhibitory effects of Tamus communis L. root extracts. Phytotherapy Research. 23(2): 283-8. Feb 2009.
